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the freeze-drying process comprises the following steps, preparing a liquid and filling at the temperature of 20-30 , putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 , preserving heat for 1h, when the temperature of a clapboard is-10 +/-2 , preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 within 1h, preserving heat for 4h, transferring to a cold trap at-50 , vacuumizing to below 10Pa, firstly reducing the temperature of the plate layer to-5 , preserving heat for 2h, then heating the plate layer to 10 within 3h, when a product is at 0 , starting to increase the temperature of the plate layer to 40 , and when the product is increased to 30 , preserving heat for 5 h

doi: 10.3389/fpls.2022.979678 114 LeeRMChoiHShinJSKimKYooKH

Astrocytic endfeet re-cover blood vessels after removal by laser ablation

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SARS-CoV-2 brain infiltration results in cerebral vascular/endothelial dysfunction with cerebral circulatory deficits such as frontotemporal hypoperfusion [25]

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