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doi: 10.1097/SPC.0b013e3282f5272e 111 SacerdoteP.FranchiS.GerraG.LecceseV.PaneraiA

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The plasmid mixture was prepared by mixing the plasmid DNA with a Lipo8000 transfection reagent in a 1:1.6 ratio, was then added into the cell plates and incubated for a duration of 24 hto allow the internalization of the plasmid DNA

However, when the cell detects an apoptotic stimulus, such as DNA damage or metabolic stress, the intrinsic apoptotic pathway is triggered and mitochondrial cytochrome c is released into the cytosol (Kroemer et al., 2007)